Article Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should Ther. Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. (1990). Environ Toxicol Pharmacol. 4h after microporation, the sizes of the micropore decreased slightly. Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. Physicochemical studies of oligodextran. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. 2009;301(8):60913. Peptide and protein transdermal drug delivery. Advances in chemistry series Washington, DC: American Chemical Society., pp. FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). 2012;87(6):1728. However, in vitro investigations are very few. Epub 2012 Oct 18. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. 8600 Rockville Pike Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. Chemical penetration enhancers for transdermal drug delivery systems. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. 4K, b M.W. Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. PubMedGoogle Scholar. Ubbelohde, Leo. An undergraduate physical chemistry experiment. Monolayers and Thin Films of Dextran Hydrophobically Modified. ; Wood, P.J. Drug deliv. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. (2007). ; Bos, G.W. In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. Provided by the Springer Nature SharedIt content-sharing initiative. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. ; Veldhuis, T.F.J. Cookies policy. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), Viswanath, D.S. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. In addition, we evaluated the regeneration of SC after microporation. 2008;28(5):64557. Drug Deliv. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. (1999). Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. Human skins (Full-thickness human skin) were supplied by HansBiomed. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. Journal of American Chemical Society, 76 (20), 5041-5052. These results are in agreement with a previous study which suggested that the microporation using radiofrequency causes disruption of the SC, enabling the percutaneous permeation of the FITC-dextran [21]. (2008). (1969). Recent Pat Drug Deliv Formul. The measurements of the transepidermal water loss (TEWL) were taken using the Tewameter (Courage-Khazaka Electronic GmbH, Cologne, Germany) to evaluate the skin barrier function after microporation. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. This situation is mainly the result of using dextans, solvent, additives, etc. Biophysical Journal, 87, 4259-4270. Ghimici, Luminita; Nichifora, Marieta; Eich, Andreas; Wolf, Bernhard A. Dextrans in Aqueous Solution. High Manufacturing Standards of Molecular Probes Dextrans. Differences were considered statistically significant at p <0.05. Masuelli, Martin A. Hydrodynamic properties of whole arabic gum. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. Keywords: FITC dextran; intestinal permeability; transit time. Based on these results a difference of approximately 1015% between the amounts of drugs permeated through the human skin and pig skin models should be considered during human clinical trials. Journal of Chemical Education, 49(8), 577-579. The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. Ortega, A.; Garca de la Torre, J. Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". Citation-(RIS Learn More. 2011;16(2):518. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. The skin poration was performed using the microporation device (LG electronics, Korea). Journal of Polymer Science, 17, 527-546. Please enable it to take advantage of the complete set of features! In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. Int J Pharm Investig. Molecular weight (M v) and hydrodynamic properties can be calculated from there. European. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the Viscosity of Liquids. Elias, Hans-Georg (1975). National Library of Medicine Granath, K. A. Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. Using the polymer standards, a plot of the ln [] vs ln Mw usually gives a straight line. Epub 2022 Feb 2. The measurements of dextrans molecular weight were determined by size exclusion HPLC (Gilson, France) with Refraction Index detector using a Polysep-GFC-P5000 column (7.8030 mm). The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. The Viscosity of Dilute Solutions of Long-Chain Molecules. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Comparison of single-point and dilution procedures. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Hydrodynamic radii ( RH) were determined by dynamic light scattering. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. Dextran produced from Weissella cibaria JAG8 was purified and characterized. Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. Springer Editions. International Journal of Biological Macromolecules, 23, 135-141. The receptor chamber was filled with PBS. (1958). eCollection 2018 Feb. Molecules. Thermo Fisher Scientific. Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with De Belder, A. N. (2001). On the molecular weight distribution of dextran T-500. Materiaux 1-5. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. 2012;29(7):201729. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. SWC designed and coordinated the research. Pharm Res. Gums and stabilisers for the Food Industry, 5, 447-450. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. J Appl Toxicol. European Polymer Journal, 43, 1744-1753. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. Catiker, E.; Guner, A. Radiofrequency with microneedling. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. Not for use in diagnostic procedures. Privacy WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. 43-1, 115-147. Carbohydr Polym. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. California Privacy Statement, Nat Biotechnol. Durand, A. Carbohydrate Research, 160, 205-214. Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. 1905/05) for their financial support. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. A solvent at this temperature is called a theta solvent [53]. Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t Thus, it is accepted that, for every flexible-chain polymer in a (ideal) solvent, there is a universal value of 0 = 2.1-2.5x1023mol-1 [52]. 2023 BioMed Central Ltd unless otherwise stated. Structure Properties of Dextran. Probiotics Antimicrob Proteins. At 25C both values of a and kM-H are within the range reported by [3, 8, 16]. government site. NCI CPTC Antibody Characterization Program. DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). Figure5 shows the cumulative amount of the FITC-dextran (M.W. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. ; Tsuchiya, H.M.; Rist, C.E. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. MeSH terms The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. Dextrans can function efficiently as anterograde or retrograde tracers, From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. (2012). 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. The human skins were from the back or thigh of a 24-year-old woman. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. Human cadaver skin has been confirmed as alive skin tissue through analysis of its morphology and enzymes, and its usefulness as an alternative skin membrane in drug permeation experiments has been verified [20]. The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. Guner, A.; Kibarer, G. (2001). Critical parameters for gel permeation
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